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KMID : 1149220150230020171
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Korean Journal of Oriental Medical Prescription
2015 Volume.23 No. 2 p.171 ~ p.187
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Effects of Sagan-tang and individual herbs on COPD Mice Model
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Han Jong-Min
Yang Won-Kyung
Kim Seung-Hyung
Park Yang-Chun
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Abstract
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Objective : This study aimed to evaluate the effects of Sagan-tang (SGT) on COPD mouse model.
Methods : The study was carried out by two ways (in vitro, in vivo). In vitro RAW264.7 cells (mouse macrophage) were used and analysed by flow cytometry, ELISA, Western blot. In vivo LPS and CSS challenged mice were used and its BALF had been analysed by cytospin image, FACS, ELISA, lung tissue by real-time PCR.
Results : In vitro, SGT maintained 80-100% rate of viablilty on 10 ~ 500 ㎍/㎖ concentration. In ELISA analysis with RAW264.7 cells, SGT significantly decreased NO over 30 ㎍/㎖. In flow cytometry, SGT 100 ㎍/㎖ dosage group displayed a tendency for decrease ROS. In Western blot analysis, SGT 100 ㎍/㎖ dosage group decreased NF-κB. In ELISA analysis, SGT significantly decreased TNF-α, IL-6 over 200 ㎍/㎖. In vivo SGT 200 ㎎/㎏ dosage group, application of SGT significantly decreased increase of neutrophils, TNF-α, IL-6 in BALF, muc5AC, TGF-β, TNF-α, expression of mRNA in lung tissue and histological lung injury.
Conclusion : This Study suggests usability of SGT for COPD patients by controlling lung tissue injury.
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KEYWORD
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Sagan-tang (shegan-tang), chronic obstructive pulmonary disease, cigarette smoke solution
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